Lactate Dehydrogenase (LDH)
| Product Name | Lactate Dehydrogenase |
| Source | Chicken Heart |
| Catalogue Number | 350-50 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 200 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4. |
| Protein | > 0.8 mg protein/mg (A280/Coomassie) |
| Specific Activity | > 200 U/mg protein |
| Contaminants | CPK: < 0.5% GLDH: < 0.1% SGOT/AST: < 0.02% SGPT/ALT: < 0.07% Protease: < 0.01% Ammonia: < 0.02 micromole/mg |
| Formulation | Lyophilized from sodium phosphate, pH 7.4. |
| Appearance | White powder |
| Reconstitution | > 1 mg/mL in phosphate buffered saline |
| Related Products | 490-20 - Alkaline Phosphatase from porcine kidney 310-30 - Alanine Aminotransferase from porcine heart 300-20 - Aspartate Aminotransferase from porcine heart |
| Storage | -20°C |
| Recertification | 3 years |
| Synonyms | LAD, LD, L-LDH, (S)-Lactate, LDH |
| Molecular Weight | ~136,700 (Huston et al. 1972) |
| CAS Number | 9001-60-9 |
| E.C. Number | 1.1.1.27 |
Lee Biosolutions produces Chicken Heart Lactate Dehydrogenase (LDH) for medical research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. Lactate Dehydrogenase catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LD can also catalyze the oxidation of hydroxybutyrate, Lactate Dehydrogenase (LDH) is occasionally called Hydroxybutyrate Dehydrogenase (HBD).
Lactate Dehydrogenase enzyme is also found in cerebrospinal fluid where high levels of lactate dehydrogenase in cerebrospinal fluid are often associated with bacterial meningitis. High levels of Lactate Dehydrogenase (LDH) enzyme can also be found in cases of viral meningitis, generally indicating the presence of encephalitis and poor prognosis.
Lee Biosolutions, Inc
