Lactate Dehydrogenase (LDH)

Product Name	Lactate Dehydrogenase
Source	Chicken Heart
Catalogue Number	350-50
Purity	Purified
Form	Lyophilized
Activity	> 200 U/mg (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein	> 0.8 mg protein/mg (A₂₈₀/Coomassie)
Specific Activity	> 200 U/mg protein
Contaminants	CPK: < 0.5% GLDH: < 0.1% SGOT/AST: < 0.02% SGPT/ALT: < 0.07% Protease: < 0.01% Ammonia: < 0.02 micromole/mg
Formulation	Lyophilized from sodium phosphate, pH 7.4.
Appearance	White powder
Reconstitution	> 1 mg/mL in phosphate buffered saline
Related Products	490-20 - Alkaline Phosphatase from porcine kidney 310-30 - Alanine Aminotransferase from porcine heart 300-20 - Aspartate Aminotransferase from porcine heart
Storage	-20°C
Recertification	3 years
Synonyms	LAD, LD, L-LDH, (S)-Lactate, LDH
Molecular Weight	~136,700 (Huston et al. 1972)
CAS Number	9001-60-9
E.C. Number	1.1.1.27