Esterase (PLE)
| Product Name | Esterase |
| Source | Porcine Liver |
| Catalogue Number | 343-10 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 75 U/mg powder |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of ethyl butyrate to butyrate and ethanol per minute at pH 8.0 and 37°C |
| Protein | > 0.4 mg protein/mg (Coomassie) |
| Specific Activity | Reported in U/mg protein |
| Formulation | Lyophilized from sodium phosphate, pH 7.5. |
| Ammonia | < 0.01 μmole/mg |
| Related Products | 127-12 - Bilirubin, Unconjugated - Indirect 651-10 - Urobilinogen 188-10 - Cholesterol Esterase from porcine pancreas |
| Storage | -20°C |
| Recertification | 3 years |
| Synonyms | Carboxylesterase, Carboxyl esterase, Carboxylic-ester hydrolase, PLE |
| Application | Asymmetric Ester Hydrolysis, Research, Life Science, Manufacturing, Urinalysis |
| Raw Material Source | United States of America (USDA licensed establishment) |
| Molecular Weight | 162,000 |
| CAS Number | 9016-18-6 |
| E.C. Number | 3.1.1.1 |
| Gene | CES1, APLE, PMPMEase, REH, TGH |
| Gene ID | 397478 |
| References | 1) A rapid oxygen exchange on prostaglandins in plasma represents plasma esterase activity that is inhibited by diethylumbelliferyl phosphate with high affinity, Rapid Communications in Mass Spectrometry, Volume 26, Issue 20, 30 October 2012, Pages 2472-2476. |
Lee Biosolutions is the largest producer of Esterase from porcine liver used in urine controls and for kinetic resolutions and asymmetric ester hydrolysis in organic chemistry.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. 250,000 kU single lot sizes.
Liver esterases are heterogeneous enzymes responsible for detoxification of xenobiotics and activation of ester and amide prodrugs. They catalyze the hydrolysis of a carboxylic esters to the corresponding alcohols and carboxylic acids. These enzymes have wide substrate specificities[i], are resistant to denaturation[ii], and retain activity under near-anhydrous conditions[iii].
Lee Biosolutions manufactures esterase from porcine (Sus scrofa, domestic pig) liver in bulk quantities for use in synthesis, preparation of clinical controls, and research.
Identification
1.Synonyms: PLE, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
2.EC #: 3.1.1.1
3.CAS #: 9016-18-6
4.Accession #: Q29550, EST_1PIG (19 – 566)
Properties
1.Molecular function: carboxylic ester hydrolase
Carboxylic ester + Water = Alcohol + Carboxylic acid
2.Inhibitors: diisopropylflurophosphate, phenylmethylsulfonyl fluoride[iv], tosyl-L-phenylalanine chloromethyl ketone[v]
3.Activator: CHAPS[vi]
4.Stability: Stable at neutral pH, frozen or lyophilized. Loses activity rapidly at pH 4.5 and belowvii
5.pH optimum: 6 – 8i,iv
6.Structure: 60,074 MW monomervi (sequence), 162,000 polymerizes readily at neutral pHvii
7.Post-translational modifications
a.Residue 80: N-linked (GlcNAc…) asparagine
b.Disulfide bonds (88 – 117 and 274 – 285)
c.Residue 379: Phosphoserine
8.Isoelectric point: 5.0vii
9.Extinction coefficient (E0.1%280 nm): 1.3[vii]
Diagnostic Relevance: Surrogate for preparation of leukocyte esterase controls or calibrators in urinalysis. Urinary leukocyte esterase is an indicator of urinary tract infection.
Other Applications: Enantioselective biocatalysis[viii],[ix],[x]
[i] www.brenda-enzymes.org
[ii] Farb, D. and Jencks, W.P., Arch. Biochem. Biophys. 203, 214-226 (1980)
[iii] Lopez, M. et al., Biophys. J. 99, L62-L64 (2010)
[iv] Junge, W. and Heymann, E., Eur. J. Biochem. 95, 519-525 (1979)
[v] Oboh, O.T. and Lamango, N.S., J. Biochem. Mol. Toxicol. 22, 51-62 (2008)
[vi] www.uniprot.org
[vii] Dudman, N.P.B and Zerner, B., Meth. Enzymol. 24 190-208 (1970)
[viii] Henke, E., et al., ChemBioChem 4, 485-493 (2003)
[ix] Sousa, H.A. et al., J. Chem. Technol. Biotechnol. 75, 707-714 (2000)
[x] Tamm, C., Pure & Appl. Chem. 64, 1187-1191 (1992)
Lee Biosolutions, Inc
