Leukocytes, Sonicated
| Product Name | Sonicated Leukocytes |
| Source | Human Leukocytes |
| Catalogue Number | 342-10 |
| Form | Liquid |
| Purity | Blood Fraction |
| Activity | 5.0 - 6.0 U/mL (Elastase) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5 (In the presence of 500 mM sodium chloride and 0.2% Triton X-100) |
| Protein | > 1.0 mg/mL (Coomassie) |
| Dipstick Assay | Leukocytes: > 2+ Protein: Negative Glucose: Negative Blood: Less than trace |
| Dipstick Protocol | 0.9 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip® 10 SG and/or Siemens Multistix® 10 SG test strips. |
| Formulation | Suspension in 154 mM sodium chloride. |
| Appearance | Milky white suspension. May form aggregates due to freeze/thaws. |
| Handling | Sonicate prior to use to disperse aggregates. |
| Related Products | 910-12 - Bilirubin Conjugate, Ditaurate, Sodium Salt 341-10 - Whole Leukocytes from human 343-10 - Esterase from porcine liver |
| Storage | -20°C |
| Recertification | 2 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | Immune cells, LE, Elastase |
Lee Biosolutions is the leading supplier of Human Sonicated Leukocytes (Elastase) for urinalysis manufacturing and research uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single lots from 5 L available.
0.9 mL per liter of urine will give a positive reaction for leukocytes with a Siemens Multistix 10 or Roche Chemstrip 10 dipstick.
Human leukocyte esterase a urine test for presence of white blood cells abnormalities associated with infection. Leukocyte urine tests reveal the presence of granulocyte esterases, which cleave a derivatized pyrazole amino acid ester to liberate derivatized hydroxy pyrazole. This pyrazole then reacts with a diazonium salt to produce a purple color.
Leukocyte is a general term for a white blood cell. Human Leukocyte(s) include lymphocytes, polymorphonuclear leukocytes and monocytes
White blood cells in the urine usually indicate a urinary tract infection. Human leukocyte esterase (LE) tests detect esterase, an enzyme released by white blood cells. Human White blood cells are produced in the bone marrow and help to defend the body against infectious disease and foreign materials as part of the immune system. Human Leukocytosis is very common in acutely ill patients. It occurs in response to a wide variety of conditions, including viral, bacterial, fungal, or parasitic infection, cancer, and exposure to certain medications or chemicals.
Lee Biosolutions, Inc
