Alpha-Amylase - DFP Treated
Catalogue Number: 122-18
Description
| Product Name | Alpha-Amylase, DFP Treated |
| Source | Porcine Pancreas |
| Catalogue Number | 122-18 |
| Purity | Purified |
| Form | Lyophilized |
| Product Details | Alpha-Amylase (122-10) was treated with DFP (diisopropylfluorophosphate) to inactivate serine proteases. |
| Activity | > 70 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C. |
| Protein | > 0.4 mg protein/mg (Coomassie) |
| Specific Activity | > 100 U/mg protein |
| Contaminants | Lipase: < 5% Protease: < 1% Ammonia: < 0.1 micromole/mg |
| Formulation | Lyophilized from 20 mM tris, 150 mM sodium chloride, and 3 mM calcium chloride, pH 7.5. |
| Appearance | Off-white to white powder |
| Related Products | 400-10 - Lipase from porcine pancreas 197-25 - Colipase from porcine pancreas 122-10 - α-Amylase from porcine pancreas |
| Storage | -20°C |
| Recertification | 3 years |
| Synonyms | 1,4-alpha-D-Glucan-glucanohydrolase, amy2, PPA, PA |
| Molecular Weight | ~54,000 |
| CAS Number | 9000-90-2 |
| E.C. Number | 3.2.1.1 |
| Gene | AMY2 |
| Gene ID | 397397 |
| Accession No | P00690 |
Applications
Overview
Lee Biosolutions produces porcine pancreatic alpha-Amylase for clinical research and diagnostic manufacturing.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Unit Definition: One unit will catalyze the hydrolysis of one micromole of maltopentaose, which through coupled reactions results in the formation of 5 micromoles of glucose=6-phosphate per minute at 37C. Measured at 340 nm as an equimolar amount of NADH produced by a coupled reaction.
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Lee Biosolutions, Inc
