Creatine Kinase MB (CK-MB)
| Product Name | Creatine Kinase MB Isoenzyme |
| Source | Human Heart |
| Catalogue Number | 190-25 |
| Purity | Purified (Control Grade) |
| Form | Lyophilized |
| Activity | > 25 U/mg solid (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. |
| Protein | > 0.25 mg protein/mg (Coomassie) |
| Specific Activity | Report in U/mg protein |
| Isozyme Content | CK-MB: > 99% (Helena QuickGel® CK) CK-MM: < 1% CK-BB: < 1% |
| Contaminants | GOT/AST: < 1% (Dimension® Clinical Chemistry System) LDH: < 1% Ammonia: < 0.1 micromole/mg solid |
| Formulation | Lyophilized from 10 mM tris-succinate with 2 mM EDTA and 2 mM DTT, pH 7.5. |
| Reconstitution | Tris buffered saline with 1% BSA, 1 mM DTT and 1 mM EDTA. |
| Appearance | Light yellow to tan powder. |
| Recertification | 3 years |
| Storage | -20°C |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | CKMB, CK-MB |
| Molecular Weight | 84,000 |
| CAS Number | 9001-15-4 |
| E.C. Number | 2.7.3.2 |
Lee Biosolutions is the leading supplier of Human Creatine Kinase MB (CK-MB) isoenzyme for diagnostic manufacturing and research purposes.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Human Creatine Kinase is one of the most important myocardial markers (in spite of not being altogether cardiac-specific), with well-established roles in confirming acute myocardial infarction (AMI). It can also used for monitoring reperfusion during thrombolytic therapy following AMI. Testing is performed when the total Human Creatine Phosphokinase (CPK) level is elevated. Isoenzyme testing can help differentiate the source of the damaged tissue. Human CPK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type). Three different isoenzymes: Creatine Kinase MM, BB, and MB isoenzymes exist.
Lee Biosolutions, Inc
