Butyrylcholinesterase (BChE)
| Product Name | Butyrylcholinesterase |
| Source | Equine Serum |
| Catalogue Number | 130-10 |
| Purity | Highly Purified |
| Form | Lyophilized |
| Activity | > 50 U/mg (Siemens Dimension® Clinical Chemistry System) |
| Unit Definition | A change of 0.2 milliabsorbance units (mA) per minute corresponds to pseudocholinesterase activity of 1 U/mL at 37 ̊C. |
| Protein | > 0.1 mg protein/mg (A280nm, E = 1.36) |
| Specific Activity | > 300 U/mg protein (Typically > 900 U/mg protein) |
| Appearance | White to pale, grey-green powder |
| Reconstitution | Reconstitute at 5 mg/mL using 50 mM Tris-HCl pH 7.3 - 7.5. |
| Solubility | Clear, colorless to slightly green solution (1 mg/mL in 0.9% saline) |
| Related Products | 135-10 - BCHE from human serum 343-10 - Esterase from porcine liver |
| Storage | -20°C |
| Recertification | 4 years |
| Synonyms | BCHE, BuChE, PCHE, pseudocholinesterase, plasma cholinesterase, Acylcholine acyl-hydrolase, Choline esterase, butyryl |
| Application | Diagnostic Manufacturing, Clinical Research |
| CAS Number | 9001-08-5 |
| E.C. Number | 3.1.1.8 |
| Gene | BCHE |
| Accession No | P06276 (human, 29 – 602), Q9N1N9 (equine) |
| Reaction | Acylcholine + H2O = choline + carboxylic acid |
| Kinetics | KM=18 µM (butyrylthiocholine, 25 degrees Celsius) |
| Activators | Ca2+ |
| Inhibitors | Hg2+, EDTA, diisopropylfluorophosphate |
| Optimum pH | 7 - 8 |
Lee Biosolutions is the leading supplier of horse serum Butyrylcholinesterase (BCHE) for research and diagnostic manufacturing.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Butyrylcholinesterase (BCHE, or BuChE), is a non-specific cholinesterase enzyme that hydrolyses many different choline esters. In humans, it is found primarily in the liver and is encoded by the BCHE gene.
It is very similar to the neuronal acetylcholinesterase, which is also known as RBC or erythrocyte cholinesterase. The term "serum cholinesterase" is generally used in reference to a clinical test that reflects levels of both of these enzymes in the blood. Assay of butyrylcholinesterase activity in plasma can be used as a liver function test as both hypercholinesterasemia and hypocholinesterasemia indicate pathological processes.
Lee Biosolutions, Inc
