Pregnant Mare Serum Gonadotropin (PMSG)
| Product Name | Pregnant Mare Serum Gonadotropin |
| Source | Equine Serum |
| Catalogue Number | 493-10 |
| Form | Lyophilized |
| Purity | Highly Purified |
| Activity | > 1,000 IU/mg powder |
| Activity | Report in IU/vial |
| Additional Info | Luteinizing hormone-like activity |
| Appearance | White to off-white solid |
| Application | Uses include inducing superovulation of laboratory animals for research or further manufacturing purposes. |
| Reconstitution | 1 mg/mL is deionized water. Further dilutions should be in a buffer such as tris buffered saline with 1% BSA as a carrier. Store at -20°C after reconstitution, avoiding repeat freeze-thaw cycles. |
| Related Products | 996-11 - FSH antigen from human pituitary gland 996-31 - LH antigen from human pituitary gland 996-51 - TSH antigen from human pituitary gland |
| Storage | 2-8°C |
| Recertification | 2 years |
| Synonyms | PMSG, eCG, Equine Chorionic Gonadotropin, Anteron; Antostab; Eleagol; Equine Cyonin; Equine Gonadotropin; Seragon; Serotropin; Predalon-S; Priatin, Gonadyl1 |
| CAS Number | 9002-70-4 |
| E.C. Number | 232-663-9 |
| Accession No | P01220 (alpha subunit, 25 – 120), P08751 (beta subunit, 21 – 169) |
| Gene | CGA, LHB |
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Pregnant mare serum gonadotropin (PMSG) is a glycoprotein hormone synthesized in the domestic horse placenta. Commercial preparations are isolated from the serum of pregnant mares. The hormone is a dimer of non-covalently associated alpha and beta subunits. It is a unique member of the gonadotropin family in that it contains high levels of FSH and LH activity[i]. Its ability to stimulate ovulation is determined in a bioassay, and the results are expressed as international units (IU)[ii]. PMSG that has been purified to homogeneity has approximately 16,000 IU/mg protein[iii]. PMSG may be used to induce ovulation in livestock prior to artificial insemination.
[i] Moore, W.T., Jr. and D.N. Ward, JBC 255, 6923 (1980)
[ii] Cole, H.H. and J. Erway, Endocrinology 29, 514 (1941)
[iii] Gospodarowicz, D. and H. Papkoff, Endocrinology 80, 699 (1967)
Research Applications
1.Induction of ovulation in experimental animals
2.Study of molecular determinants of LH and FSH activities
Properties (Equus caballus, horse)
1.Molecular function: hormone activity, expressed in IU
2.Structure: 64,030 MW heterodimer[i]
a.Alpha chain: 43,720 MW
b.Beta chain: 16,960 MW
3.Post-translational modification:
a.Alpha chain N-linked (GlcNAc…) asparagine, approximately 21% of mass, interchain disulfides
b.Beta chain O-linked (GalNAc…) serine and threonine, N-linked (GlcNAc…) asparagine, approximately 46% of mass, intrachain disulfides
4.Beta E0.1% = 0.238 (sequence)
[i] Christakos, S. and O.P. Bahl, JBC 254, 4253 (1979)
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