Aspartate Aminotransferase (AST, GOT)

Product Name	Aspartate Aminotransferase
Source	Porcine Heart
Catalogue Number	300-20
Purity	Purified
Form	Lyophilized
Activity	> 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
Protein	> 0.7 mg protein/mg solid (A₂₈₀) Report mg protein/mg solid (Coomassie)
Specific Activity	> 110 U/mg protein (A₂₈₀) Report U/mg protein (Coomassie)
Contaminants	CPK: < 0.1% GPT/ALT: < 0.1% LDH: < 0.1% GLDH: < 0.1% MDH: < 0.01% Protease: < 0.1% Ammonia: < 0.005 micromole/mg
Formulation	Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0.
Appearance	Yellow to yellow-green powder
Reconstitution	> 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0.
Isoform	Cytosolic
Related Products	480-20 - Alkaline Phosphatase from calf intestine 310-30 - Alanine Aminotransferase from porcine heart 350-50 - Lactate Dehydrogenase from chicken heart
Storage	-20°C
Recertification	3 years
Application	Control, Calibrator, Research, Manufacturing
Synonyms	Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase
Molecular Weight	~92,000 (Feliss and martinez-Carrion 1970)
CAS Number	9000-97-9
E.C. Number	2.6.1.1
Gene	GOT1
Accession No	P00503